RRThis field contains the abstract of an article.
RRYY1 is ubiquitously expressed zinc finger DNA binding protein.
It can act as a transcriptional repressor or activator and, when
binding at the initiator element, as a component of the basal
transcription complex. Binding sites for YY1 have been reported
in a wide variety of promoters and they exhibit substantial
diversity in their sequence. To better understand how YY1 interacts
with DNA and to be able to predict the presence of YY1 sites in
a more comprehensive fashion, we have selected YY1 binding sites
from a random pool of oligonucleotides. The sites display considerable
heterogeneity, but contain a conserved 5'-CAT-3' core flanked by
variable regions, generating the consensus
5'-(C/g/a)(G/t)(G/t/a)CATN(T/a)(T/g/c)-3', where the upper case
letters represent the preferred base. This high degree of flexibility
in DNA recognition can be predicted by modeling the interaction
of the four YY1 zinc fingers with DNA and a detailed model for
this interaction is presented and discussed.