In this section, the user enters in line
DNA/protein Binding data for each sequence variant, estimating the relative degree of protein binding on a scale of +1 (maximal) to 1 (minimal). In our example, it was experimentally determined that the gel mobility shift assay of the GpIbb gene allele WT contains a band corresponding to the DNA complex with unknown nuclear protein, whereas in case of -133C/G allele, this band is absent. There is an illustration how these experimental data are entered into the proper boxes of the field DNA-protein binding: i.e., the first allele, WT, was assigned the input value +1, and, the second allele, -133C/G, the value 0. Then the button Calculate should be clicked, following which the calculated values will appear in the window Prediction.