Internet resource for optimization of transgene expression in plants

Please input the nucleotide sequence of transgenic mRNA. 5'-UTR: from 5' mRNA end till AUG codon ( not included; 5'-truncated sequences can be used ) CDS: protein coding sequence ( from AUG codon to stop codon ), 3'-UTR: (starting immediately downstream of stop codon till the poly(A) tail beginning; 3'-truncated variants can be used ). Please do not use intron-containg sequences.

Enter 5'UTR (from screen or from file):


Enter CDS (from screen or from file):


Enter 3'UTR (from screen or from file):

Programs for prediction of mRNA translational activity:

Features available:
1. Optimization of synonymous codon content
2. Information on species-specific translation initiation signal
3. Information on species-specific translation termination signal

References on the related resources (further they will be integrated into this server):

1. Leader_RNA: Prediction of translation initiation rate basing on the transgene 5'-UTR sequence features in dicot plants, monocot plants, mammals.

2. TRSIG: prediction of putative post-transcriptional regulatory elements within transgene 5'-UTR or 3'-UTR (for the prediction, use BLAST search on the TRSIG www-page).